PhageLine unpacks the science of bacteriophages, one paper at a time. From groundbreaking studies to surprising discoveries, we translate complex research into engaging conversations. Whether you are steeped in microbiology or just discovering the fascinating world of phages, PhageLine delivers a unique perspective on the science behind these bacterial predators.PhageLine is on <a href="https://bsky.app/profile/phageline.bsky.social" rel="nofollow">BlueSky</a>. Follow us never to miss anything again.This podcast is generated using artificial intelligence. Infectious disease scientists review the scripts before production.

https://s3.us-east-2.amazonaws.com/pod-public-media-files/2025/10/12/16/4e0fae04-b6a8-4c75-b485-cf4a54179231_ep32.mp3

Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss the effect of an antibiotic and a probiotic on phage communities in swine gut microbiota as featured in a recent paper by <a href="https://doi.org/10.1186/s13104-025-07484-w" rel="nofollow">Lemieux et al. in BMC Research Notes</a>. Have fun!This episode was generated using artificial intelligence.

PhageLine #32: Antibiotic and Probiotic Effects on the Gut Phageome

https://s3.us-east-2.amazonaws.com/pod-public-media-files/2025/10/2/13/5bee5c96-71c7-41ea-90a7-0aa7f8fd14c7_ep31.mp3

Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss Vitamin B12 release through bacteriophage-mediated cell lysis of a marine bacterium as featured in a recent paper by <a href="https://doi.org/10.1093/ismeco/ycaf136" rel="nofollow">Sultana et al. in ISME Communications</a>. Have fun!This episode was generated using artificial intelligence.

PhageLine #31: Bacteriophage-Mediated Vitamin B12 Release

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss the synergistic effect of bacteriophages and fusidic acid against meticillin-­resistant Staphylococcus pseudintermedius in an ex vivo canine dermis model as featured in a recent paper by <a href="https://doi.org/10.1111/vde.70030" rel="nofollow">Ehling et al. in Veterinary Dermatology</a>. Have fun!This episode was generated using artificial intelligence.

PhageLine #30: Bacteriophage-Fusidic Acid Synergy In An Ex Vivo Dermis Model

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss Bacteriophage immunotherapy with artificial antigen-­directed immune labeling, as presented in a recent paper by <a href="https://doi.org/10.1126/sciadv.adr1911" rel="nofollow">Yao et al. in Science Advances</a>. Have fun!This episode was generated using artificial intelligence.

PhageLine #29: Phage Therapy With Artificial Antigen-­Directed Immune Labeling

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss Phage quest: a beginner’s guide to explore viral diversity in the prokaryotic world which is a recent paper by <a href="https://doi.org/10.1093/bib/bbaf449" rel="nofollow">Wendling et al. in Briefings in Bioinformatics</a>. Have fun!This episode was generated using artificial intelligence.

PhageLine #28: A Beginner’s Guide to Viral Diversity

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss the anti-restriction functions of injected phage proteins, as presented in a recent paper by <a href="https://doi.org/10.1038/s41467-025-63056-3" rel="nofollow">Silas et al. in Nature Communications</a>. Have fun!This episode was generated using artificial intelligence.

PhageLine #27: Anti-Restriction Phage Proteins

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss the in vitro and in vivo selection and cost of bacteriophage resistance on natural Escherichia coli, as presented in a recent paper by <a href="https://doi.org/10.1093/femsml/uqaf017" rel="nofollow">Cardoso et al. in FEMS microLife</a>. Have fun!This episode was generated using artificial intelligence.

PhageLine #26: Selection and Cost of Bacteriophage Resistance

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Hello and welcome to PhageLine - your line to everything phageThis week, we will discuss the efficacy of sequential phage-colistin therapy against carbapenem-resistant Acinetobacter baumannii to overcome phage resistance, as presented in a recent paper by <a href="https://doi.org/10.1128/spectrum.00855-25" rel="nofollow">Choi et al. in Microbiology Spectrum</a>. Have fun!This episode was generated using artificial intelligence.

PhageLine #25: Efficacy of Sequential Phage-Colistin Therapy

https://s3.us-east-2.amazonaws.com/pod-public-media-files/2025/8/9/21/eaae1a18-36a4-4f24-ab2c-a1e31d0c4ea9_ep24.mp3

Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss a hydrogel with physically entrapped phages to control Pseudomonas aeruginosa infections in burn wound models, as presented in a recent paper by <a href="https://doi.org/10.1016/j.bioadv.2025.214403" rel="nofollow">Walji et al. in Biomaterials Advances</a>. Have fun! This episode was generated using artificial intelligence.

PhageLine #24: A Phage Hydrogel for Burn Wounds

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss a bacteriophage‑based virus‑like particle vaccine that induces cross‑reactive neutralising antibodies against porcine epidemic diarrhoea viruses (PEDV) by talking about a recent paper from <a href="https://doi.org/10.1186/s13567-025-01559-z" rel="nofollow">Gu et al in Veterinary Research</a>. Have fun! This episode was generated using artificial intelligence.

PhageLine #23: Bacteriophage Vaccine Against PEDV

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss Phage-antibiotic synergy to combat multidrug-resistant strains of Gram-negative ESKAPE pathogens by examining a recent paper by <a href="https://doi.org/10.1038/s41598-025-01489-y" rel="nofollow">Morris et al. in Scientific Reports</a>. Have fun! This episode was generated using artificial intelligence. An infectious disease researcher reviewed the episode&#39;s contents.

PhageLine #22: Phage-Antibiotic Synergy

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss the impact of microplastics on bacteriophage activity in aqueous environments by talking about a recent paper from <a href="https://doi.org/10.1021/acs.jpcb.5c01316" rel="nofollow">Ochirbat et al. in the Journal of Physical Chemistry</a>. Have fun! This episode was generated using artificial intelligence. An infectious disease researcher reviewed the episode&#39;s contents.

PhageLine #21: Microplastics and Bacteriophages

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss the bacteriophage potential of SARS-CoV-2 in human gut microbiota by talking about a recent paper by <a href="https://doi.org/10.12688/f1000research.109236.2" rel="nofollow">Petrillo et al in F1000</a>. Have fun! This episode was generated using artificial intelligence. An infectious disease researcher reviewed the episode&#39;s contents.

PhageLine #20: Is SARS-CoV-2 a Bacteriophage?

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss a method for the targeted discovery of bacteriophages by co-culture, called Phage DisCo, which was recently published in <a href="https://doi.org/10.1128/msystems.01644-24" rel="nofollow">mSystems by Eleanor Rand and her colleagues at Harvard University</a>. Usually, I would say &#34;have fun&#34; here; however, there is also a dark side to this episode: all five federal grants that supported different parts of this work were part of the mass termination of Harvard grants three weeks ago, as confirmed by principal investigator Michael Baym. This episode was generated using artificial intelligence. An infectious disease researcher reviewed the contents of the episode.

PhageLine #19: Targeted Discovery by Co-Culture (Phage DisCo)

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss the in vitro genomic characterization and pre-clinical evaluation of a new thermostable lytic Obolenskvirus phage formulated as a hydrogel against carbapenem-resistant Acinetobacter baumannii by discussing a recent paper by <a href="https://doi.org/10.1038/s41598-025-99788-x" rel="nofollow">Sherif et al. in Scientific Reports</a>. Have fun! This episode was generated using artificial intelligence. An infectious disease researcher reviewed the contents of the episode.

PhageLine #18: A Highly Robust Acinetobacter baumannii Phage

https://s3.us-east-2.amazonaws.com/pod-public-media-files/2025/5/17/20/ce3f83f0-c343-4d77-86e4-e46f08a8f44d_ep17.mp3

Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss personalized inhaled bacteriophage therapy for treating multidrug-resistant Pseudomonas aeruginosa in cystic fibrosis by talking about a recent publication by <a href="https://doi.org/10.1038/s41591-025-03678-8" rel="nofollow">Chan et al in Nature Medicine</a>. Have fun! This episode was generated using artificial intelligence. An infectious disease researcher reviewed the contents of the episode.

PhageLine #17: Personalized Phage Therapy in Cystic Fibrosis Infections

https://s3.us-east-2.amazonaws.com/pod-public-media-files/2025/5/10/16/af3cf55b-a22a-4625-9fd6-5c48e436eac4_ep16.mp3

Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss the effects of Phage–phage competition and biofilms on interactions between two virulent bacteriophages and Pseudomonas aeruginosa, which were recently published by <a href="https://doi.org/10.1093/ismejo/wraf065" rel="nofollow">Bürkle et al. in ISME</a>. Have fun! This episode was generated using artificial intelligence. An infectious disease researcher reviewed the contents of the episode.

PhageLine #16: Bacteriophage Interactions

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Hello and welcome to PhageLine - your line to everything phage.This week, we will discuss <a href="https://fitted.ceb.uminho.pt/phagedpo" rel="nofollow">PhageDPO</a>, a machine-learning-based computational framework for identifying phage depolymerases, recently published by <a href="https://doi.org/10.1016/j.compbiomed.2025.109836" rel="nofollow">Viera et al. in Elsevier&#39;s Computers in Biology and Medicine</a>. Have fun! This episode was generated using artificial intelligence. An infectious disease researcher reviewed the contents of the episode.

PhageLine #15: Identifying Phage Depolymerases

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Hello and welcome to PhageLine - your line to everything phage.This week, we will focus on the differences in phage recognition and immunogenicity in humans unraveled in a <a href="https://doi.org/10.1002/eji.202451543" rel="nofollow">recent paper by Le et al. in the European Journal of Immunology</a>. Have fun! This episode was generated using artificial intelligence. An infectious disease researcher reviewed the contents of the episode.

PhageLine #14: Phage Recognition and Immunogenicity in Humans

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Hello and welcome to PhageLine - your line to everything phage.This week, we will focus on optimizing phage engineering through an accelerated evolution platform by discussing a recent paper by <a href="https://doi.org/10.1038/s41598-020-70841-1" rel="nofollow">Favor et al. from Nextbiotics Inc. in Scientific Reports</a>. Have fun! This episode was generated using artificial intelligence. An infectious disease researcher reviewed the contents of the episode.

PhageLine #13: Optimizing Bacteriophage Engineering Through an Accelerated Evolution Platform

Your line to everything phage

PhageLine unpacks the science of bacteriophages, one paper at a time. From groundbreaking studies to surprising discoveries, we translate complex research into engaging conversations. Whether you are steeped in microbiology or just discovering the fascinating world of phages, PhageLine delivers a unique perspective on the science behind these bacterial predators.

PhageLine is on BlueSky (@phageline.bsky.social). Follow us never to miss anything again.

This podcast is generated using artificial intelligence. Infectious disease scientists review the scripts before production.